Hi,
Can you check if the "Microsoft Exchange" certificate has been selected on the Exchange Backend website on https 444 binding on both the mailbox servers?
What happens if you try logging with https://localhost/ecp on the mailbox server?
Since you have re-created the ECP virtual directory, can you check the authentication are set as the default?
https://learn.microsoft.com/en-us/exchange/clients/default-virtual-directory-settings?view=exchserver-2019
Event description which has been mentioned is for source ".NET" or "Exchange"? If so, could you please share the complete error
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