Hello,
- MFA server 8.0.1 works with Windows Server 2016.
- The safest approach would be to add new MFA Servers to the existing collection and turn off older MFA servers.
- That would be the best in tandem with previous recommendation.
- Please follow steps detailed in Back up and restore Azure MFA Server.
Please let me know if you need more help. If the answer was helpful to you, please accept it and, optionally, provide feedback so that other members in the community can benefit from it.