This should work:
lblTotal.Text = "0"
For intCount = 0 To lstCost.Items.Count - 1
lblTotal.Text += +lstCost.Items(intCount)
Next
But if you add the ‘Option Strict On’ line to the beginning of your file to make more reliable programs, then consider this alternative too:
Dim sum As Integer = 0
For Each item In lstCost.Items
Dim n As Integer
If Integer.TryParse(item.ToString, n) Then sum += n
Next
lblTotal.Text = sum.ToString
or something like this:
lblTotal.Text = lstCost.Items.Cast(Of Object) _
.Sum(Function(item)
Dim n As Integer
Integer.TryParse(item.ToString, n)
Return n
End Function).ToString