Hello @Amar-Azure-Practice if you want to limit the scale to one instance only then you could modify the App Setting WEBSITE_MAX_DYNAMIC_APPLICATION_SCALE_OUT.
However, if your code is asynchronous, the same app instance can process multiple requests in parallel.
You may also check the Singleton attribute concept here.
https://github.com/Azure/azure-functions-host/issues/912
Please let me if this helps.