If you are also interested in the problems of your first approach, then consider the next aspects.
If you want to change the variable, use assignment:
litem = Regex.Replace(litem, . . .
If you want to find any of listed characters, use “[…]”.
Make sure that the characters, which also have a special meaning in regular expressions, are preceded with “\”. Something like this:
litem = Regex.Replace(litem, "['/.,<>=+|\-)( :;~!@#$%\^&*_?[\]"" ]", "").ToUpper()