Hello @Alex ,
Thank you for posting here.
We can check whether the IP address of the problematic VM machine belongs to the new subnet we moved to.
If the IP address of the problematic VM machine is not in the new subnet we moved to, maybe the VM machine may take time to find a DC in other site to authenticate, and it is looking for the proper DC to authenticate when the ad-logons begins.
Also, we can check whether the DNS server of the problematic VM machine points to the correct DNS server after moving the problematic VM machine to the new subnet.
And check whether we have changed any settings after move the VM to different subnets (such as we may change the IP address of the VM), and check whether there are any restrictions on network access.
Best Regards,
Daisy Zhou
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