[Paper] Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry
Great to see this paper on Quantum Dots in Nature Protocols by May Wang and Shuming Nie...along w/ the other authors from GaTech and Emory.
I've been tracking their work and it really seems promising on helping to get to the goal of personalized medicine.
Bioconjugated quantum dots (QDs) provide a new class of biological labels for evaluating biomolecular signatures (biomarkers) on intact cells and tissue specimens. In particular, the use of multicolor QD probes in immunohistochemistry is considered one of the most important and clinically relevant applications. At present, however, clinical applications of QD-based immunohistochemistry have achieved only limited success. A major bottleneck is the lack of robust protocols to define the key parameters and steps. Here, we describe our recent experience, preliminary results and detailed protocols for QD–antibody conjugation, tissue specimen preparation, multicolor QD staining, image processing and biomarker quantification. The results demonstrate that bioconjugated QDs can be used for multiplexed profiling of molecular biomarkers, and ultimately for correlation with disease progression and response to therapy. In general, QD bioconjugation is completed within 1 day, and multiplexed molecular profiling takes 1–3 days depending on the number of biomarkers and QD probes used.
Cross Posted from Dan Fay's Blog (http://blogs.msdn.com/dan\_fay)